Isolation and characterization of mutations affecting UDPG pyrophosphorylase activity in Dictyostelium discoideum.
نویسندگان
چکیده
Dimond, R. L., Loomis, W. F. "Acetylglucosaminidase mutants in Dictyostelium discoideum." 1973 Genetics. 74: 64 Cardelli, J. A., Dimond, R. L. "Effect of Starvation and Cell-Cell Interaction on Protein Synthesis in Dictyostelium-discoideum." The 20th Annual Meeting of the American Society for Cell Biology, Cincinnati, Ohio, USA, 1980, J. Cell Biology, 87: (2 Part 2) 275A Knecht, D. A., Dimond, R. L. "Synthesis and Processing of Lysosomal Enzymes in Dictyostelium-discoideum." 1980 The 20th annual meeting of the American Society for Cell Biology, USA, Nov. 14-18, J Cell Biol. 87 (2 part 2) Livi, George, Dimond, Randall L. "Mutants that affect α-mannsoidase-1 regulation in Dictyostelium discoidem." 1982 Meeting of the Genetics Society of America, USA, June 14-27, Genetics 100 (1 Part 2)
منابع مشابه
THE STRUCTURAL GENE FOR a-MANNOSIDASE-1 IN DICTYOSTELIUM DISCOIDEUM
We have isolated 4 independent mutations affecting a-mannosidase-1, a a developmentally regulated activity in Dictyostelium discoideum. Three of these result in a thermolabile a-mannosidase-1 activity. One mutation also affects the substrate affinity (Km) of the activity. In diploids these mutations show a gene dosage effect and are all alleles. The structural gene for a-mannosidase-I, as defin...
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The stability of uridine diphosphoglucose pyrophosphorylase was examined in extracts prepared at different stages of development in Dictyostelium discoideum. In the early stages, the kinetics of inactivation were nonlinear, and, therefore, it was not possible to determine the specific enzyme activity. In the later stages of development, the enzyme was stable, but it could be rapidly inactivated...
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The pathway of ribosomal RNA biogenesis in Dictyostelium discoideum has been defined through identification, isolation, and characterization of the rapidly labeled nuclear RNAs which are intermediates in the process. Comparison of the methylation patterns, base compositions, two-dimensional oligonucleotide maps, and hybridization properties of these intermediate RNAs with those of mature rRNAs ...
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Three independent mutations affecting N-acetylglucosaminidase in Dictyostelium discoideum were mapped by the parasexual system and found to lie on linkage group IV. These mutations as well as two others were found to be recessive and noncomplementing in heterozygous diploids. Thus they all appear to affect the nagA locus. Since two of the mutations give rise to thermolabile enzyme, this defines...
متن کاملMetabolism of the cellular slime mould Dictyostelium discoideum grown in axenic culture.
1. The DNA, RNA, protein and carbohydrate contents of myxamoebae of Dictyostelium discoideum strain Ax-2 were measured after growth on bacteria or in various axenic media. 2. Myxamoebae grown in the different axenic media have similar DNA, RNA and protein contents, but there are marked differences in the contents of glycogen and free sugars. The DNA and protein contents of myxamoebae grown on b...
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عنوان ژورنال:
- Developmental biology
دوره 50 1 شماره
صفحات -
تاریخ انتشار 1976